Publications

2005

• Observation of sub-100 ps conformational changes in photolyzed carbonmonoxy-myoglobin probed by time-resolved circular dichroism
  
  • Dartigalongue Thibault
  • Hache François
  Chemical Physics Letters, Elsevier, 2005, 415 (4-6), pp.313-316. A time-resolved circular dichroism (CD) experiment is carried out on carbonmonoxy-myoglobin. The CD is measured with a sub-picosecond time resolution after ligand dissociation and the data are interpreted thanks to a classical CD calculation based on the polarizability theory. We observe a decrease of the CD signal in a few picoseconds and a sub-100 ps relaxation towards steady-state deoxy-myoglobin values which we assign to a stress of the proximal histidine which relaxes with the global reorganization of the protein from its liganded geometry to its deliganded one. 2005 Elsevier B.V. All rights reserved (10.1016/j.cplett.2005.09.022)
  DOI : 10.1016/j.cplett.2005.09.022
• Functionality of nitrated acetylcholine receptor: The two-step formation of nitrotyrosines reveals their differential role in effectors binding
  
  • Négrerie Michel
  • Martin Jean-Louis
  • Nghiêm H.-O.
  FEBS Letters, Wiley, 2005, 579 (12), pp.2643. The presence of nitrotyrosines is associated with several neurodegenerative pathologies. We evaluated the functionality of the nicotinic acetylcholine receptor possessing nitrotyrosines. The spectrum of the nitrated receptor displays an absorption band characteristic of ortho-nitrophenol. The presence of carbamylcholine in the agonist site prevented the effect of nitration by tetranitromethane in some conditions. The nitration occurred with two discrete steps and pointed out the differential involvement of tyrosines in the binding of acetylcholine and neurotoxin. We concluded that at least two residues involved in agonist binding can be nitrated, which bring similar contributions to the binding energy of the neurotransmitter. Cop. 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. (10.1016/j.febslet.2005.03.084)
  DOI : 10.1016/j.febslet.2005.03.084
• Mid-infrared electric field characterization using a visible charge-coupled-device-based spectrometer
  
  • Kubarych Kevin J.
  • Joffre Manuel
  • Moore Amy
  • Belabas Nadia
  • Jonas David
  Optics Letters, Optical Society of America - OSA Publishing, 2005, 30 (10), pp.1228-1230. We characterize ultrashort mid-infrared pulses through upconversion by using the stretched pulses obtained from the uncompressed output of a chirped-pulse amplifier. The power spectrum thus translated into the visible region can be readily measured with a standard silicon CCD camera-based spectrometer. The spectral phase is also characterized by a variant of zero-added-phase spectral phase interferometry for direct electric field reconstruction. This is a general method that provides a multiplex advantage over conventional infrared detector array-based methods. © 2005 Optical Society of America (10.1364/OL.30.001228)
  DOI : 10.1364/OL.30.001228
• Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy: erratum
  
  • Pena Ana-Maria
  • Strupler Mathias
  • Boulesteix Thierry
  • Godeau G.
  • Schanne-Klein Marie-Claire
  Optics Express, Optical Society of America - OSA Publishing, 2005, 13 (17), pp.6667. We present corrected versions of the list of authors and of Section 2.1. (10.1364/OPEX.13.006667)
  DOI : 10.1364/OPEX.13.006667
• Activationless electron transfer through the hydrophobic core of cytochrome c oxidase
  
  • Jasaitis Audrius
  • Rappaport F.
  • Pilet Eric
  • Liebl Ursula
  • Vos Marten H.
  Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2005, 102 (31), pp.10882. Electron transfer (ET) within proteins occurs by means of chains of redox intermediates that favor directional and efficient electron delivery to an acceptor. Individual ET steps are energetically characterized by the electronic coupling V, driving force ΔG, and reorganization energy λ. λ reflects the nuclear rearrangement of the redox partners and their environment associated with the reactions; λ ≈ 700-1,100 meV (1 eV = 1.602 × 10-19 J) has been considered as a typical value for intraprotein ET. In nonphotosynthetic systems, functional ET is difficult to assess directly. However, using femtosecond flash photolysis of the CO-poised membrane protein cytochrome c oxidase, the intrinsic rate constant of the low-ΔG electron injection from heme a into the heme a 3-CuB active site was recently established at (1.4 ns)-1. Here, we determine the temperature dependence of both the rate constant and ΔG of this reaction and establish that this reaction is activationless. Using a quantum mechanical form of nonadiabatic ET theory and common assumptions for the coupled vibrational modes, we deduce that λ is <200 meV. It is demonstrated that the previously accepted value of 760 meV actually originates from the temperature dependence of CuB-CO bond breaking. We discuss that low-ΔG, low-λ reactions are common for efficiently channeling electrons through chains that are buried inside membrane proteins. (10.1073/pnas.0503001102)
  DOI : 10.1073/pnas.0503001102
• Etudes spectroscopiques du senseur à oxygène FixL
  
  • Jasaitis Audrius
  • Vos Marten H.
  • Balland Véronique
  • Mattioli T. A.
  • Bouzhir-Sima Latifa
  • Liebl Ursula
  , 2005, pp.poster.
• Sudden polarisation and coherent vibration in bacteriorhodopsin
  
  • Groma Geza
  • Colonna Anne
  • Joffre Manuel
  • Vos Marten H.
  • Martin Jean-Louis
  , 2005.
• Ligand transfer dynamics in cytochrome c oxidase using infrared femtosecond spectroscopy
  
  • Treuffet Johanne
  • Ogilvie Jennifer P.
  • Polack Thomas
  • Pilet Eric
  • Lambry Jean-Christophe
  • Joffre Manuel
  • Vos Marten H.
  • Martin Jean-Louis
  • Alexandrou Antigoni
  , 2005.
• Téléphonie cellulaire et cancer: où en est-on? [Cellular phones and cancer: Current status]
  
  • Colonna Anne
  Bulletin du Cancer, Elsevier, 2005, 92 (7), pp.637. L'évaluation de l'impact des nouvelles technologies sur le corps humain est indispensable pour imposer les normes réglementaires limitant les risques sanitaires. L'apparition et l'évolution des téléphones cellulaires ont été parmi les plus rapides dans l'histoire des innovations. De nombreuses recherches ont été effectuées pour tenter d'évaluer le lien éventuel entre effet sur la santé et téléphonie mobile, au niveau du téléphone cellulaire mais également des stations de base. Cet article présente un aperçu des connaissances en matière de recherche sur l'incidence des ondes radiofréquences. Études épidémiologiques, cellulaires et animales se succèdent mais aucune conclusion définitive n'a pu être établie. Même si certains effets biologiques sur des cellules en culture ont été observés, le lien avec un possible développement de cancer chez l'homme reste encore loin d'être établi. Les études animales montrent pour la plupart des résultats négatifs. Quant aux études épidémiologiques, elles manquent d'un recul suffisant pour pouvoir évaluer l'effet des technologies utilisées aujourd'hui. Face à l'inquiétude des populations, opérateurs de téléphonie mobile, constructeurs et instances gouvernementales financent nombre de projets scientifiques afin de permettre une réglementation adaptée et efficace. [Evaluation of the impact of new technologies on the human body is essential in order to impose regulations to limit health risks. The appearance and evolution of cellular phones have been one of the fastest in the history of innovation. Research reported worldwide has tried to evaluate any potential link between adverse health effects and the mobile phone and its broadcasting stations. This article gives an overview of current research knowledge on the impact of radiofrequency waves on health. Epidemiologic, cellular and animal studies have been carried out, but none of them have reached definitive conclusions. Although some biological effects on cell culture have been observed, their link with human cancer development is far from established. Most of the animal studies show negative results. Epidemiologic studies lack a sufficient perspective to be able to evaluate the effect of evolving technologies used today. High levels of concern by the public have urged mobile phone operators, manufacturers and governmental authorities to finance a number of scientific projects aimed at defining adapted and effective regulations.] Cop. John Libbey Eurotext.
• Nonlinear optical spectroscopy of chiral molecules
  
  • Fischer Peer
  • Hache François
  Chirality, Wiley, 2005, 17 (8), pp.421. We review nonlinear optical processes that are specific to chiral molecules in solution and on surfaces. In contrast to conventional natural optical activity phenomena, which depend linearly on the electric field strength of the optical field, we discuss how optical processes that are nonlinear (quadratic, cubic, and quartic) functions of the electromagnetic field strength may probe optically active centers and chiral vibrations. We show that nonlinear techniques open entirely new ways of exploring chirality in chemical and biological systems: The cubic processes give rise to nonlinear circular dichroism and nonlinear optical rotation and make it possible to observe dynamic chiral processes at ultrafast time scales. The quadratic second-harmonic and sum-frequency-generation phenomena and the quartic processes may arise entirely in the electric-dipole approximation and do not require the use of circularly polarized light to detect chirality. They provide surface selectivity and their observables can be relatively much larger than in linear optical activity. These processes also give rise to the generation of light at a new color, and in liquids this frequency conversion only occurs if the solution is optically active. We survey recent chiral nonlinear optical experiments and give examples of their application to problems of biophysical interest. Cop. 2005 Wiley-Liss, Inc. (10.1002/chir.20179)
  DOI : 10.1002/chir.20179
• Ultrafast polarization and coherent charge motions in bacteriorhodopsin observed by infrared emission interferometry
  
  • Colonna Anne
  • Lambry Jean-Christophe
  • Groma Geza
  • Vos Marten H.
  • Martin Jean-Louis
  • Joffre Manuel
  , 2005.
• Toxines et douleurs
  
  • Bon Cassian
  • Goudey-Perriere F.
  • Goyffon Max
  • Sauviat Martin-Pierre
  , 2005, pp.238. Malgré les progrès récents réalisés au cours de la dernière décennie, les mécanismes cellulaires et les structures moléculaires impliqués dans la perception, la transmission et l'intégration des sensations douloureuses restent encore largement inconnus. D'un autre point de vue, des observations récentes ont démontré que des toxines issues d'organismes variés, et pour certaines connues depuis longtemps pour agir sur la propagation ou la transmission des influx nerveux, possèdent des propriétés antalgiques. L'identification de ces toxines actives contre la douleur est loin d'être achevée et leur inventaire se poursuit avec beaucoup de succès. Par ailleurs, les cibles moléculaires et les mécanismes d'action de ces toxines analgésiantes commencent à être identifiés. Elles apparaissent ainsi comme des outils précieux pour analyser et caractériser les structures moléculaires impliquées dans la perception et la transmission de la douleur. Certaines de ces toxines analgésiantes sont même à l'origine de médicaments puissants et originaux utilisables pour atténuer voire supprimer la sensibilité à la douleur. Sans prétendre pour autant couvrir la totalité de ce vaste sujet, le cinquième volume des Rencontres en Toxinologie illustre, au moyen de quelques exemples précis, l'ensemble de cette problématique et témoigne de la vitalité de la recherche, notamment française, dans ce domaine
• Third harmonic generation microscopy for the velocimetric analysis of Drosophila embryo development
  
  • Débarre Delphine
  • Supatto Willy
  • Farge Emmanuel
  • Moulia Bruno B.
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  , 2005.
• Multiphoton microscopy of unstained living cardiac and vascular tissue
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Pagès Nicole
  • Senni Karim
  • Godeau Gaston
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  , 2005.
• Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy
  
  • Pena Ana-Maria
  • Strupler Mathias
  • Boulesteix Thierry
  • Schanne-Klein Marie-Claire
  Optics Express, Optical Society of America - OSA Publishing, 2005, 13 (16), pp.6268-6274. We recorded one-photon excited fluorescence (1PEF) and two-photon excited fluorescence (2PEF) spectra of purified keratin from human epidermis, and determined the action cross section of this endogenous chromophore. We used this spectroscopic analysis to analyse multiphoton images of skin biopsies and assign the intrinsic fluorescence signals in the epidermis. We observed a good agreement between in situ and in vitro 2PEF spectra of keratin. This study provides a comprehensive characterization of the 2PEF signal of the keratins from the epidermis, and will be of practical interest for multiphoton imaging of the skin. © 2005 Optical Society of America (10.1364/OPEX.13.006268)
  DOI : 10.1364/OPEX.13.006268
• Nonlinear spectroscopy of chiral molecules
  
  • Fischer P.
  • Hache François
  Chirality, Wiley, 2005, 17, pp.421-437.
• Etudes fonctionnelles du senseur à oxygène bactérien Dos
  
  • Bouzhir-Sima Latifa
  • Jasaitis Audrius
  • Vos Marten H.
  • Liebl Ursula
  , 2005.
• 11th Congress of the European Society for Photobiology
  
  • Dartigalongue Thibault
  • Hache François
  , 2005.
• Dynamique ultrarapide au sein des protéines vue par spectroscopie optique: interactions hémoprotéines-ligands
  
  • Vos Marten H.
  , 2005.
• Functionalized luminescent oxide nanoparticles for single Na+ channel imaging
  
  • Casanova Didier
  • Giaume D.
  • Beaurepaire Emmanuel
  • Sauviat Martin-Pierre
  • Auksorius Egidijus
  • Buissette V.
  • Lahlil K.
  • Martin Jean-Louis
  • Gacoin Thierry
  • Boilot Jean-Pierre
  • Alexandrou Antigoni
  , 2005.
• Geminate carbon monoxide rebinding to a c-type haem
  
  • Silkstone G.
  • Jasaitis Audrius
  • Vos Marten H.
  • Wilson M.
  Dalton Transactions, Royal Society of Chemistry, 2005, 21 (7), pp.3489. A chemically modified form of cytochrome c (cyt. c), termed carboxymethyl cytochrome c (cm cyt. c), possesses a vacant sixth coordination site to the haem iron that is available to bind external ligands. We present data on the rapid flash photolysis of CO from the ferrous haem iron of cm cyt. c and describe the kinetics and spectral transitions that accompany the recombination. This was achieved using 30-femtosecond laser pulses and a white light continuum to monitor spectral transitions. Whereas the photo-dissociation quantum yield is close to 1, the yield of CO escape from the protein (the apparent quantum yield, φ) relative to myoglobin (φ = 1) is small due to rapid geminate recombination of CO. On ligand photo-dissociation the haem undergoes a spin-state transition from low-spin ferrous CO bound to penta-coordinate high-spin. Subsequently the system reverts to the CO bound form. The data were fitted with a minimum number of exponentials using global analysis. Recombination of CO with the haem iron of cm cyt. c is multiphasic (τ = 16 ps, 120 ps and 1 ns), involving three spectrally distinct components. The fraction of haem (0.11) not recombining with CO within 4 ns is similar to the value of φ (0.12) measured on the same preparation by the "pulse method" (M. Brunori, G. Giacometti, E. Antonini and J. Wyman, Proc. Natl. Acad. Sci. USA, 1973, 70, 3141-3144, ). This implies that no further geminate recombination occurs at t > 4 ns. This unusually efficient CO-haem geminate recombination indicates the sterically hindered ("caged") nature of the distal haem pocket in cm cyt. c from which it is difficult for CO to escape. The large geminate phase may be contrasted with the behaviour of myoglobin in which geminate recombination is small. This is in general agreement with the well-documented extensive structural dynamics in myoglobin that allow ligand passage, and a higher structural rigidity in cyt. c imposed by the restraints of minimising reorganisation energy for electron transfer (M. Brunori, D. Bourgeois and D. Vallone, J. Struct. Biol., 2004, 147, 223-234, ). The high pH ferrous form of cm cyt. c is a low-spin species having a lysine bound to the central iron atom of the haem (M. Brunori, M. Wilson and E. Antonini, J. Biol. Chem., 1972, 247, 6076-6081; G. Silkstone, G. Stanway, P. Brzezinski and M. Wilson, Biophys. Chem., 2002, 98, 65-77, ). This high pH (pH 8) form of deoxy cm cyt. c undergoes photo-dissociation of lysine (although the proximal histidine is possible) after photo-excitation. Recombination occurs with a time constant (τ) of 7 ps. This is similar to that observed for the geminate rebinding of the Met80 residue in native ferrous cyt. c (τ 6 ps) following its photo-dissociation (S. Cianetti, M. Negrerie, M. Vos, J.-L. Martin and S. Kruglik, J. Am. Chem. Soc., 2004, 126, 13932-13933; W. Wang, X. Ye, A. Demidov, F. Rosca, T. Sjodin, W. Cao, M. Sheeran and P. Champion, J. Phys. Chem., 2000, 104, 10789-10801, ). (10.1039/B508183C)
  DOI : 10.1039/B508183C
• Calculation of the circular dichroism spectra of carbon monoxy- and deoxy myoglobin: interpretation of a time-resolved circular dichroism experiment.
  
  • Dartigalongue Thibault
  • Hache François
  The Journal of Chemical Physics, American Institute of Physics, 2005, 123 (18), pp.184901. A calculation of the circular dichroism (CD) spectra of carbon monoxy- and deoxy myoglobin is carried out in relation with a time-resolved CD experiment. The calculation is based on the polarizability theory and the parameters are adjusted to fit the experimental absorption and CD spectra. By performing the calculation for intermediate configurations of the protein, we are able to propose an explanation of the CD structure observed on a sub-100 ps time scale. The role of the proximal histidine is, in particular, clearly demonstrated in the first step of the myoglobin relaxation from its liganded to it deliganded form. (10.1063/1.2041467)
  DOI : 10.1063/1.2041467
• Retinal isomerization dynamics in dry bacteriorhodopsin films
  
  • Colonna Anne
  • Groma Geza I.
  • Vos Marten H.
  Chemical Physics Letters, Elsevier, 2005, 415 (1-3), pp.69-73. The primary photoprocesses in neutral and acid forms of oriented dried bacteriorhodopsin films were investigated by femtosecond absorption spectroscopy. The excitation energy dependence of the signals was used to distinguish photochemistry from processes involving photophysics of photocycle intermediates. Both the kinetics and the quantum yield of all-trans excited state decay by retinal photoisomerization and subsequent J → K transition were found to be very similar as in hydrated environments. Therefore, unlike slower photocycle phases, communication of the retinal with the environment does not play a role in retinal isomerization. Our results are important for understanding recent nonlinear optical applications of such films. (10.1016/j.cplett.2005.08.132)
  DOI : 10.1016/j.cplett.2005.08.132
• Unstained Drosophila embryo development analysed by velocimetric third harmonic generation microscopy
  
  • Débarre Delphine
  • Supatto Willy
  • Farge Emmanuel
  • Moulia Bruno B.
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  , 2005.
• Sensibilité structurale et sources de contraste en microscopie par génération de troisième harmonique (THG) appliquée à la biologie
  
  • Débarre Delphine
  • Supatto Willy
  • Pena Ana-Maria
  • Fabre Aude
  • Tordjmann T.
  • Combettes Laurent
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  , 2005.