Publications

2009

• Single europium-doped nanoparticles measure temporal pattern of reactive oxygen species production inside cells
  
  • Casanova Didier
  • Bouzigues Cédric
  • Nguyên Thanh-Liêm
  • Ramodiharilafy Rivo O.
  • Bouzhir-Sima Latifa
  • Gacoin Thierry
  • Boilot Jean-Pierre
  • Tharaux Pierre-Louis
  • Alexandrou Antigoni
  Nature Nanotechnology, Nature Publishing Group, 2009, 4 (9), pp.581. Low concentrations of reactive oxygen species, notably hydrogen peroxide (H 2 O 2), mediate various signalling processes in the cell. Production of these signals is highly regulated and a suitable probe is needed to measure these events. Here, we show that a probe based on a single nanoparticle can quantitatively measure transient H 2 O 2 generation in living cells. The Y 0.6 Eu 0.4 VO 4 nanoparticles undergo photoreduction under laser irradiation but re-oxidize in the presence of oxidants, leading to a recovery in luminescence. Our probe can be regenerated and reliably detects intracellular H 2 O 2 with a 30-s temporal resolution and a dynamic range of 1-45?M. The differences in the timing of intracellular H 2 O 2 production triggered by different signals were also measured using these nanoparticles. Although the probe is not selective towards H 2 O 2, in many signalling processes H 2 O 2 is, however, the dominant oxidant. In conjunction with appropriate controls, this probe is a powerful tool for unravelling pathways that involve reactive oxygen species. Cop. 2009 Macmillan Publishers Limited. All rights reserved. (10.1038/nnano.2009.200)
  DOI : 10.1038/nnano.2009.200
• Measurement of the Second-Order Hyperpolarizability of the Collagen Triple Helix and Determination of Its Physical Origin
  
  • Deniset-Besseau Ariane
  • Duboisset Julien
  • Benichou Emmanuel
  • Hache François
  • Brevet Pierre-Francois
  • Schanne-Klein Marie-Claire
  Journal of Physical Chemistry B, American Chemical Society, 2009, 113 (40), pp.13437-13445. We performed Hyper-Rayleigh Scattering (HRS) experiments to measure the second-order nonlinear optical response of the collagen triple helix and determine the physical origin of second harmonic signals observed in collagenous tissues. HRS experiments yielded a second-order hyperpolarizability of 1.25 x 10(-27) esu for rat-tail type I collagen, a Surprisingly large value considering that collagen presents no strong harmonophore in its amino acid sequence. Polarization-resolved experiments showed intramolecular coherent contributions to the HRS signal along with incoherent contributions that are the only contributions for molecules with dimensions much smaller than the excitation wavelength. We therefore modeled the effective second-order hyperpolarizability of the 290 nm long collagen triple helix by summing coherently the nonlinear response of well-aligned moieties along the triple helix axis. This model was confirmed by HRS measurements after denaturation of the collagen triple helix and for a collagen-like short model peptide [(Pro-Pro-Gly)(10)](3). We concluded that the large collagen nonlinear response originates in the tight alignment of a large number of small and weakly efficient harmonophores, presumably the peptide bonds, resulting in a coherent amplification of the nonlinear signal. (10.1021/jp9046837)
  DOI : 10.1021/jp9046837
• Femtosecond spectroscopy from the perspective of a global multidimensional response function
  
  • Nuernberger Patrick
  • Lee Kevin F.
  • Joffre Manuel
  Accounts of Chemical Research, American Chemical Society, 2009, 42 (9), pp.1433-1441. At the microscopic level, multidimensional response functions, such as the nonlinear optical susceptibility or the time-ordered response function, are commonly used tools in nonlinear optical spectroscopy for determining the nonlinear polarization resulting from an arbitrary excitation. In this Account, we point out that the approach successfully developed for the nonlinear polarization can also be used in the case of a directly observable macroscopic quantity. This observable can be, for example, the electric field radiated in a nonlinear mixing experiment, the rate of fluorescence resulting from one- or two-photon absorption, or the rate of a photochemical reaction. For each of these physical processes, perturbation theory can be used to expand the measured quantity in a power series of the exciting field, and an appropriate global response function can be introduced for each order of perturbation. At order n, the multidimensional response function will depend on n variables (either time or frequency) and have the same general properties as the nonlinear susceptibility resulting, for example, from time invariance or causality. The global response function is introduced in this Account in close analogy with the nonlinear susceptibility or the time-ordered microscopic response. We discuss various applications of the global response function formalism. For example, it can be shown that in the weak field limit, a stationary signal induced in a time-invariant system is independent of the spectral phase of the exciting field. Although this result had been demonstrated previously, the global response function enables its derivation in a more general way because no specific microscopic model is needed. Multidimensional spectroscopy is obviously ideally suited to measure the global multidimensional response function. It is shown that the second (or third)-order response can be exactly measured with 2D (or 3D) spectroscopy by taking into account the exact shape of the exciting pulses. In the case of a 2D measurement of the third-order response, a particular projection of the complete 3D response function is actually measured. This projection can be related to a mixed time and frequency representation of the response function when the pulses are assumed to be infinitely short. We thus show that the global response function is a useful tool for deriving general results and that it should help in designing future experimental schemes for femtosecond spectroscopy. Cop. 2009 American Chemical Society. (10.1021/ar900001w)
  DOI : 10.1021/ar900001w
• Inferring maps of forces inside cell membrane microdomains
  
  • Masson J.-B.
  • Casanova Didier
  • Türkcan Silvan
  • Voisinne G.
  • Popoff Michel
  • Vergassola M.
  • Alexandrou Antigoni
  Physical Review Letters, American Physical Society, 2009, 102 (4), pp.48103. Mapping of the forces on biomolecules in cell membranes has spurred the development of effective labels, e.g., organic fluorophores and nanoparticles, to track trajectories of single biomolecules. Standard methods use particular statistics, namely the mean square displacement, to analyze the underlying dynamics. Here, we introduce general inference methods to fully exploit information in the experimental trajectories, providing sharp estimates of the forces and the diffusion coefficients in membrane microdomains. Rapid and reliable convergence of the inference scheme is demonstrated on trajectories generated numerically. The method is then applied to infer forces and potentials acting on the receptor of the toxin labeled by lanthanide-ion nanoparticles. Our scheme is applicable to any labeled biomolecule and results show its general relevance for membrane compartmentation. Cop. 2009 The American Physical Society. (10.1103/PhysRevLett.102.048103)
  DOI : 10.1103/PhysRevLett.102.048103
• Application of time-resolved circular dichroism to the study of conformational changes in photochemical and photobiological processes
  
  • Hache François
  Journal of Photochemistry and Photobiology A: Chemistry, Elsevier, 2009, 204 (2-3), pp.137-143. Circular dichroism is known to be a very sensitive probe of the molecular conformation and implementation of this technique in a pump-probe experiment is very appealing to access information on the dynamics of conformational changes occurring in photochemical or photobiological processes. In the past years, we have developed such techniques in various ways and applied them to several chemical or biological studies which are presented in this article. Applications concern spectroscopic studies of the excited state in ruthenium tris(bipyridyl) or tris(phenanthroline), dynamics of conformational changes in photoexcited binaphthol and study of the conformational changes occurring in photolyzed carboxy-myoglobin. Extension of these techniques towards biological issues such as protein folding is discussed. Cop. 2009 Elsevier B.V. All rights reserved. (10.1016/j.jphotochem.2009.03.012)
  DOI : 10.1016/j.jphotochem.2009.03.012
• Multimodal Multiphoton Imaging of Human Eye Tissues
  
  • Aptel Florent
  • Olivier Nicolas
  • Deniset Ariane
  • Plamann Karsten
  • Denis P.
  • Legeais Jean-Marc
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  Investigative Ophthalmology & Visual Science, Association for Research in Vision and Ophthalmology, 2009, 50, pp.E-Abstract 3693. Purpose:To evaluate three combined modalities of multiphoton microscopy, second-harmonic generation (SHG), third-harmonic generation (THG), and two-photon-excited fluorescence (2PEF) for imaging cornea and trabecular meshwork in human intact eye tissue. Methods:A tunable femtosecond laser chain ( = 700-1250 nm) comprising a titanium-sapphire laser oscillator and an optical parametric oscillator was used to produce 2PEF (380-620nm), SHG (/2=430 or 600nm) and THG (/3= 400nm). Eight corneoscleral discs from eye bank and seven fresh corneal buttons obtained after penetrating keratoplasty were examined with water-immersion objectives. Forward and backward signals were detected and compared. Results:The three imaging modalities provide complementary information on intact tissue over the entire thickness of the cornea. THG imaging reveals the tissue morphology, including the epithelium structure with sub-cellular resolution. Polarization-resolved THG microscopy reveals stromal birefringent domains. In phenol-stained corneas, THG also reveals the keratocytes network. SHG imaging probes the distribution of stromal collagen lamellas organization. 2PEF imaging reveals the elastic component of the extra-cellular matrix and the distribution of fluorescent organelles (i.e. mitochondria) in stromal and epithelial cells. The trabeculum images show the three-dimensional organisation of the trabecular lamellas. Emission is predominantly forward directed for THG and SHG but in some cases, images can be recorded in the epi-direction. Conclusions:The combined imaging modalities of SHG, THG, and 2PEF microscopy are effective methods to evaluate cornea and trabecular meshwork microstructures in situ. This imaging approach should prove particularly appropriate for assessing corneal and glaucoma physiopathology, and might be amenable to in vivo diagnostics.
• Suppression of perturbed free-induction decay and noise in experimental ultrafast pump-probe data
  
  • Nuernberger Patrick
  • Lee Kevin F.
  • Bonvalet Adeline
  • Polack Thomas
  • Vos Marten H.
  • Alexandrou Antigoni
  • Joffre Manuel
  Optics Letters, Optical Society of America - OSA Publishing, 2009, 34 (20), pp.3226-3228. We apply a Fourier filtering technique for the global removal of coherent contributions, like perturbed freeinduction decay, and noise, to experimental pump-probe spectra. A further filtering scheme gains access to spectra otherwise only recordable by scanning the probe's center frequency with adjustable spectral resolution. These methods cleanse pump-probe data and allow improved visualization and simpler analysis of the contained dynamics. We demonstrate these filters using visible pump/mid-infrared probe spectroscopy of ligand dissociation in carboxyhemoglobin. Cop. 2009 Optical Society of America. (10.1364/OL.34.003226)
  DOI : 10.1364/OL.34.003226
• Heme ligand binding properties and intradimer interactions in the full-length sensor protein Dos from Escherichia coli and its isolated heme domain
  
  • Lechauve C.
  • Bouzhir-Sima Latifa
  • Yamashita Taku
  • Marden M.C.
  • Vos Marten H.
  • Liebl Ursula
  • Kiger L.
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (52), pp.36146. Dos from Escherichia coli is a bacterial gas sensor protein comprising a heme-containing gas sensor domain and a phosphodiesterase catalytic domain. Using a combination of static light scattering and gel filtration experiments, we established that, as are many other sensor proteins, the full-length protein is dimeric. The full-length dimer (association constant <10 nm) is more stable than the dimeric heme domain (association constant ∼1 μm), and the dimer interface presumably includes both sensor and catalytic domains. Ultrafast spectroscopic studies showed little influence of the catalytic domain on kinetic processes in the direct vicinity of the heme. By contrast, the properties of ligand (CO and O2) binding to the heme in the sensor domain, occurring on a microsecond to second time scale, were found to be influenced by (i) the presence of the catalytic domain, (ii) the dimerization state, and in dimers, (iii) the ligation state of the other subunit. These results imply allosteric interactions within dimers. Steady-state titrations demonstrated marked cooperativity in oxygen binding to both the full-length protein and the isolated heme domain, a feature not reported to date for any dimeric sensor protein. Analysis of a variety of time-resolved experiments showed that Met-95 plays a major role in the intradimer interactions. The intrinsic binding and dissociation rates of Met-95 to the heme were modulated ∼10-fold by intradimer and sensor-catalytic domain interactions. Dimerization effects were also observed for cyanide binding to the ferric heme domains, suggesting a similar role for Met-95 in ferric proteins. (10.1074/jbc.M109.066811)
  DOI : 10.1074/jbc.M109.066811
• Removing cross-phase modulation from midinfrared chirped-pulse upconversion spectra
  
  • Lee Kevin F.
  • Nuernberger Patrick
  • Bonvalet Adeline
  • Joffre Manuel
  Optics Express, Optical Society of America - OSA Publishing, 2009, 17 (21), pp.18738-18744. We observe that narrow spectral features in mid-infrared spectra obtained by chirped-pulse up-conversion are strongly distorted by crossphase modulation between the mid-infrared field and the chirped pulse. We discuss the consequences of this effect on spectral resolution, and introduce a correction method that recovers masked lines. This simple correction can be applied either when the upconverted field is fully characterized, such as in multidimensional spectroscopy, or when causality can be used, such as in absorption spectroscopy, which we demonstrate experimentally. Cop.2009 Optical Society of America. (10.1364/OE.17.018738)
  DOI : 10.1364/OE.17.018738
• Femto-second ultrashort laser wakefield electron bunch-duration measurements: a prism-based dispersion visible-to-IR spectrometer
  
  • Lim J.
  • Faure Jérôme
  • Gallot Guilhem
  • Lundh O.
  • Rechatin C.
  • Malka Victor
  , 2009, pp.735919. (10.1117/12.829134)
  DOI : 10.1117/12.829134
• Extended fano model of extraordinary electromagnetic transmission through subwavelength hole arrays in the terahertz domain
  
  • Masson Jean-Baptiste
  • Podzorov Alexander
  • Gallot Guilhem
  Optics Express, Optical Society of America - OSA Publishing, 2009, 17 (17), pp.15280-15291. We developed an extended Fano model describing the Extraordinary Electromagnetic Transmission (EET) through arrays of subwavelength apertures, based on terahertz transmission measurements of arrays of various hole size and shapes. Considering a frequency-dependent coupling between resonant and non-resonant pathways, this model gives access to a simple analytical description of EET, provides good agreement with experimental data, and offers new parameters describing the influence of the hole size and shape on the transmitted signal. Cop. 2009 Optical Society of America. (10.1364/OE.17.015280)
  DOI : 10.1364/OE.17.015280