Publications

2006

• La transglutaminase tissulaire promeut l'organisation fibrillaire du collagène et la progression de la fibrose rénale hypertensive dépendante de l'angiotensine II. (sélectionné pour le prix poster)
  
  • Hernest Monica
  • Pena Ana-Maria
  • Beaurepaire Emmanuel
  • Chatziantoniou C.
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  , 2006.
• True near field versus contrast near field imaging.
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14 (24), pp.11566-11574. We demonstrate that in near field imaging, interaction between light and sample can be divided into two main areas: the true near field and the contrast near field domain. We performed extensive numerical simulations in order to identify the limits of these areas, and to investigate contrast near field imaging in which much easier propagation calculation can be achieved. Finally, we show an application with terahertz axonal imaging. © 2006 Optical Society of America (10.1364/OE.14.011566)
  DOI : 10.1364/OE.14.011566
• Ultrafast Conformational Changes in Carboxy-Myoglobin Studied by Time-Resolved Circular Dichroism
  
  • Dartigalongue Thibault
  • Hache François
  , 2006.
• A Hydrophobic Distal Pocket Affects NO-heme Geminate Recombination Dynamics in Dehaloperoxidase and H64V Myoglobin
  
  • Franzen Stefan
  • Jasaitis Audrius
  • Belyea Jennifer
  • Brewer Scott
  • Casey Romain
  • Macfarlane a W Iv
  • Stanley R.
  • Vos Marten H.
  • Martin Jean-Louis
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110, pp.14483-14493.
• Time-resolved circular dichroism in carbonmonoxy-myoglobin: The central role of the proximal histidine
  
  • Dartigalongue Thibault
  • Hache François
  Chirality, Wiley, 2006, 18 (4), pp.273. A calculation of the circular dichroism (CD) spectra of carbonmonoxy-and deoxy-myoglobin is carried out in relation to a time-resolved CD experiment. This calculation allows us to assign a dominant role to the proximal histidine in the definition of the electronic normal modes and to interpret the transient CD structure observed in a strain of the proximal histidine. This strain builds up in 10 ps and relaxes in 50 ps as the protein evolves towards its deoxy form. Cop. 2006 Wiley-Liss, Inc. (10.1002/chir.20254)
  DOI : 10.1002/chir.20254
• Dynamics of NO rebinding to the heme domain of NO synthase-like proteins from bacterial pathogens
  
  • Gautier Christian
  • Martasek P.
  • Mikula I.
  • Nioche P.
  • Raman C.S.
  • Slama-Schwok Anny
  Nitric Oxide, 2006, 15 (4), pp.312. Some Gram-positive bacterial pathogens harbor a gene that encodes a protein (HNS, Heme domain of NO Synthase-like proteins) with striking sequence identity to the oxygenase domain of mammalian NO synthases (NOS). However, they lack the N-terminal and the Zn-cysteine motif participating to the stability of an active dimer in the mammalian isoforms. The unique properties of HNS make it an excellent model system for probing how the heme environment tunes NO dynamics and for comparing it to the endothelial NO synthase heme domain (eNOSHD) using ultrafast transient spectroscopy. NO rebinding in HNS from Staphylococcus aureus (SA-HNS) is faster than that measured for either Bacillus anthracis (BA-HNS) or for eNOSHD in both oxidized and reduced forms in the presence of arginine. To test whether these distinct rates arise from different energy barriers for NO recombination, we measured rebinding kinetics at several temperatures. Our data are consistent with different barriers for NO recombination in SA-HNS and BA-HNS and the presence of a second NO-binding site. The hypothesis that an additional NO-binding cavity is present in BA-HNS is also consistent with the effect of the NO concentration on its rebinding. The lack of the effect of NO concentration on the geminate rebinding in SA-HNS could be due to an isolated second site. We confirm the existence of a second NO site in the oxygenase domain of the reduced eNOS as previously hypothesized [A. Slama-Schwok, M. Négrerie, V. Berka, J.C. Lambry, A.L. Tsai, M.H. Vos, J.L. Martin, Nitric oxide (NO) traffic in endothelial NO synthase. Evidence for a new NO binding site dependent on tetrahydrobiopterin? J. Biol. Chem. 277 (2002) 7581-7586]. This site requires the presence of arginine and BH4; and we propose that NO dynamic and escape from eNOS is regulated by the active site H-bonding network connecting between the heme, the substrate, and cofactor. Cop. 2006 Elsevier Inc. All rights reserved. (10.1016/j.niox.2006.03.010)
  DOI : 10.1016/j.niox.2006.03.010
• Interferometric Fourier transform coherent anti-stokes Raman scattering
  
  • Cui M.
  • Joffre Manuel
  • Skodack J.
  • Ogilvie Jennifer P.
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14 (18), pp.8448-8458. We present an interferometric time-domain Fourier transform implementation of coherent anti-Stokes Raman scattering (CARS). Based on a single femtosecond laser source, the method provides a straight-forward scheme for obtaining high resolution CARS spectra. We give a theoretical description of the method, and demonstrate good agreement between simulation and experimental CARS spectra. We also discuss the method's relation to other CARS approaches for microscopy and microspectroscopy applications. Cop. 2006 Optical Society of America. (10.1364/OE.14.008448)
  DOI : 10.1364/OE.14.008448
• Generation and complete characterization of intense mid-infrared ultrashort pulses
  
  • Ventalon Cathie
  • Fraser James M.
  • Likforman Jean-Pierre
  • Villeneuve D. M.
  • Corkum Paul B.
  • Joffre Manuel
  Journal of the Optical Society of America B, Optical Society of America, 2006, 23, pp.332.
• SHG microscopy. Quantitative scoring of kidney collagen fibrosis. (prix du meilleur poster)
  
  • Strupler Mathias
  • Hernest Monica
  • Pena Ana-Maria
  • Martin Jean-Louis
  • Beaurepaire Emmanuel
  • Tharaux Pierre-Louis
  • Schanne-Klein Marie-Claire
  , 2006.
• Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy.
  
  • Débarre Delphine
  • Supatto Willy
  • Pena Ana-Maria
  • Fabre Aurelie J
  • Tordjmann Thierry
  • Combettes Laurent
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  Nature Methods, Nature Publishing Group, 2006, 3 (1), pp.47-53. Lipid bodies have an important role in energy storage and lipid regulation. Here we show that lipid bodies are a major source of contrast in third-harmonic generation (THG) microscopy of cells and tissues. In hepatocytes, micrometer-sized lipid bodies produce a THG signal 1-2 orders of magnitude larger than other structures, which allows one to image them with high specificity. THG microscopy with approximately 1,200 nm excitation can be used to follow the distribution of lipid bodies in a variety of unstained samples including insect embryos, plant seeds and intact mammalian tissue (liver, lung). We found that epi-THG imaging is possible in weakly absorbing tissues because bulk scattering redirects a substantial fraction of the forward-generated harmonic light toward the objective. Finally, we show that the combination of THG microscopy with two-photon and second-harmonic imaging provides a new tool for exploring the interactions between lipid bodies, extracellular matrix and fluorescent compounds (vitamin A, NADH and others) in tissues. (10.1038/nmeth813)
  DOI : 10.1038/nmeth813
• Sensitivity of cardiac background inward rectifying K+ outward current (IK1) to the alkaloids lepadiformines A, B, and C
  
  • Sauviat Martin-Pierre
  • Vercauteren J.
  • Grimaud N.
  • Jugé M.
  • Nabil M.
  • Petit J.-Y.
  • Biard J.-F.
  Journal of Natural Products, American Chemical Society, 2006, 69 (4), pp.558. Three marine alkaloids, purified from Clavelina moluccensis, were structurally identified as lepadiformines A, B, and C and studied on frog atrial myocytes IK1, using the patch-clamp technique. Lepadiformine A (0.4 to 3.3 μM) blocked IK1 dose-dependently with an apparent dissociation constant (KD) equal to 1.42 μM and a stoichiometry of 0.77. The block is voltage-dependent, suggesting that lepadiformine A occupies a receptor site located at about two-thirds of the membrane depth. The shortening of the aliphatic chain at position C13 of lepadiformine B decreased the potency of the molecule to block IK1 but not the affinity (KD = 1.56 μM) and stoichiometry (0.72). Additional deletion of the oxygenated side chain at C2 in lepadiformine C markedly decreased the inhibitory effect of the molecule. In conclusion, lepadiformine modulates IK1 response in cardiac muscle. The oxygenated side chain in C2 is implicated in the affinity of lepadiformine, which behaved as an amine, for a receptor located near or inside the IK1 pore, and the aliphatic chain length at position C13 is involved in the degree of IK1 blockage. (10.1021/np050215s)
  DOI : 10.1021/np050215s
• Role of heme iron coordination and protein structure in the dynamics and geminate rebinding of nitric oxide to the H93G myoglobin mutant: Implications for nitric oxide sensors
  
  • Négrerie Michel
  • Kruglik Sergei G.
  • Lambry Jean-Christophe
  • Vos Marten H.
  • Martin Jean-Louis
  • Franzen S.
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281, pp.10389. The influence of the heme iron coordination on nitric oxide binding dynamics was investigated for the myoglobin mutant H93G (H93G-Mb) by picosecond absorption and resonance Raman time-resolved spectroscopies. In the H93G-Mb, the glycine replacing the proximal histidine does not interact with the heme iron so that exogenous substituents like imidazole may coordinate to the iron at the proximal position. Nitrosylation of H93G-Mb leads to either 6- or 5-coordinate species depending on the imidazole concentration. At high concentrations, (imidazole)-(NO)-6-coordinate heme is formed, and the photoinduced rebinding kinetics reveal two exponential picosecond phases (~10 and ~100 ps) similar to those of wild type myoglobin. At low concentrations, imidazole is displaced by the trans effect leading to a (NO)-5-coordinate heme, becoming 4-coordinate immediately after photolysis as revealed from the transient Raman spectrum. In this case, NO rebinding kinetics remain bi-exponential with no change in time constant of the fast component whose amplitude increases with respect to the 6-coordinate species. Bi-exponential NO geminate rebinding in 5-coordinate H93G-Mb is in contrast with the single-exponential process reported for nitrosylated soluble guanylate cyclase (Negrerie, M., Bouzhir, L., Martin, J. L., and Liebl, U. (2001) J. Biol. Chem. 276, 46815-46821). Thus, our data show that the iron coordination state or the heme iron out-of-plane motion are not at the origin of the bi-exponential kinetics, which depends upon the protein structure, and that the 4-coordinate state favors the fast phase of NO geminate rebinding. Consequently, the heme coordination state together with the energy barriers provided by the protein structure control the dynamics and affinity for NO-binding enzymes. Cop. 2006 by The American Society for Biochemistry and Molecular Biology, Inc. (10.1074/jbc.M513375200)
  DOI : 10.1074/jbc.M513375200
• A filtering procedure for systematic removal of pump-perturbed polarization artifacts
  
  • Polack Thomas
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14, pp.5823.
• Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity
  
  • Hunt D.E.
  • Klepac-Ceraj V.
  • Acinas S.G.
  • Bertilsson S.
  • Gautier Christian
  • Polz M.F.
  Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (3), pp.2221. The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. Copyright Cop. 2006, American Society for Microbiology. All Rights Reserved. (10.1128/AEM.72.3.2221-2225.2006)
  DOI : 10.1128/AEM.72.3.2221-2225.2006
• Terahertz achromatic quarter-wave plate
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Optics Letters, Optical Society of America - OSA Publishing, 2006, 31 (2), pp.265. Phase retardera usually present a strong frequency dependence. We discuss the design and characterization of a terahertz achromatic quarter-wave plate. This wave plate is made from six birefringent quartz plates precisely designed and stacked together. Phase retardation has been measured over the whole terahertz range by terahertz polarimetry. This achromatic wave plate demonstrates a huge frequency bandwidth (?max/?min ˜ 7), and therefore can be applied to terahertz time domain spectroscopy and polarimetry. Cop. 2006 Optical Society of America. (10.1364/OL.31.000265)
  DOI : 10.1364/OL.31.000265
• Observer les neurones grâce aux ondes térahertz
  
  • Gallot Guilhem
  La Recherche, Sciences et avenir, 2006, 397, pp.26. Des chercheurs de l'École polytechnique ont développé un dispositif d'imagerie des neurones, fondée sur l'utilisation d'ondes térahertz [1]. Ils observent ainsi les mouvements d'ions et d'eau à l'intérieur et à proximité des neurones.
• Role of Heme Iron Coordination and Protein Structure in the Dynamics and Geminate Rebinding of Nitric Oxide to H93G Myoglobin : Implications for NO-Sensors
  
  • Négrerie Michel
  • Kruglik Sergei
  • Lambry Jean-Christophe
  • Vos Marten H.
  • Martin Jean-Louis
  • Franzen Stefan
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281, pp.10389-10398.
• Functional implications of the propionate 7 Arginine 220 interaction in the FixLH oxygen sensor from Bradyrhizobium japonicum
  
  • Balland Véronique
  • Bouzhir-Sima Latifa
  • Anxolabéhère-Mallart E.
  • Boussac A.
  • Vos Marten H.
  • Liebl Ursula
  • Mattioli T.
  Biochemistry, American Chemical Society, 2006, 45, pp.2072-2084.
• Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure
  
  • Boulesteix Thierry
  • Pena Ana-Maria
  • Pagès Nicole
  • Godeau G.
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Cytometry Part A, Wiley, 2006, 69A (1), pp.20-26. We characterize the application of multiphoton microscopy to the observation of the extracellular matrix of fresh unstained vessels. Combined two-photon-excited fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable specificity. We present elastin/collagen imaging in unstained rat vessels at both micrometer and whole vessel scales, and we characterize the optical properties of rat carotid artery and aorta walls. We apply this method to evidence deleterious effects of residual doses of a pesticide on the vessel wall. This study illustrates the potential of 2PEF/SHG microscopy for pharmacological studies in unlabeled arteries. © 2005 Wiley-Liss, Inc. (10.1002/cyto.a.20196)
  DOI : 10.1002/cyto.a.20196
• Molécules d'origine naturelle et nouveaux médicaments anticancéreux [Molecules of natural origin and new anticancer drugs]
  
  • Pages N.
  • Goudey-Perriere Françoise
  • Sauviat Martin-Pierre
  • Benoit Evelyne
  • Chamorro G.
  , 2006, pp.89-98.
• Chiroptical effects in the second harmonic generation from collagens I and IV: applications in nonlinear microscopy
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Nonlinear optics, quantum optics, Old City Pub., 2006, 35 (1-3), pp.235-240. An emerging application of multiphoton microscopy is the observation of unstained intact biological tissues based on intrinsic sources of nonlinear signals. However, reliable biomedical imaging requires a thorough analysis of these endogenous signals. Looking at skin biopsies, we focused on 2-Photon Excited Fluorescence (2PEF) arising from cytokeratins in the epidermis, and second harmonic generation (SHG) from collagen fibers in the dermis. We determined the 2PEF excitation spectrum and action cross section of purified keratins from human epidermis, and obtained a good agreement with in situ measurements. In order to analyze the role of chirality and collagen type in SHG signal, we performed polarization-resolved surface SHG experiments on thin films of collagens I and IV molecules. We observed that collagen I and IV molecules exhibit comparable SHG signals, except for the chiral contributions which are absent for collagen IV and represent typically 50% of the signal for collagen I. We concluded that the large collagen I SHG efficiency is dominated by coherent effects due to the high density and quasi-crystalline order in collagen fibrils, whereas the lack of any alignment within the collagen IV molecules explains the absence of signal from this collagen type in SHG microscopy.
• Role of distal arginine in early sensing intermediates in the heme domain of the oxygen sensor FixL
  
  • Jasaitis Audrius
  • Hola Klara
  • Bouzhir-Sima Latifa
  • Lambry Jean-Christophe
  • Balland Véronique
  • Vos Marten H.
  • Liebl Ursula
  Biochemistry, American Chemical Society, 2006, 45, pp.6018-6026.