Publications

2006

• Parametric cascade downconverter for intense ultrafast mid-infrared generation beyond the Manley-Rowe limit
  
  • Fraser James M.
  • Ventalon Cathie
  Applied optics, Optical Society of America, 2006, 45 (17), pp.4109-4113. We propose a scheme to generate intense, ultrafast mid-infrared pulses with conversion efficiencies exceeding the upper bound for single-stage difference-frequency mixing as predicted by the Manley-Rowe relations. Finite-element fast Fourier transform simulations of the mixing process show that the parametric cascade downconverter generates 1.7 times more photons (at 10 μm ) than in the initial pump pulse (center wavelength of 1.48 μm , duration of 130 fs , and pulse energy of 50 μJ ), with negligible pulse spatial and temporal distortion. © 2006 Optical Society of America (10.1364/AO.45.004109)
  DOI : 10.1364/AO.45.004109
• Manipulation of morphogenetic movements in live Drosophila embryos using femtosecond pulses
  
  • Supatto Willy
  • Débarre Delphine
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Farge Emmanuel
  • Beaurepaire Emmanuel
  , 2006.
• Second harmonic generation by collagens I and IV: chiroptical and structural effects
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Schanne-Klein Marie-Claire
  , 2006.
• Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia
  
  • Gautier Clément
  • van Faassen E.
  • Mikula I.
  • Martasek P.
  • Slama-Schwok Anny
  Biochemical and Biophysical Research Communications, Elsevier, 2006, 341 (3), pp.816-821. In this work, we demonstrate that endothelial nitric oxide synthase is capable of anoxic reduction of nitrite anions to nitric oxide at physiological pH by absorption and EPR spectroscopy and electrochemical measurements. The nitrite reduction is achieved at the oxygenase domain of the protein and proceeds even in the absence of the tetrahydrobiopterin cofactor. The nitrite pathway increases by sixfold the NO production with respect to the regular arginine pathway under hypoxia, which is largely blocked. Therefore, basal levels of NO release could be sustained by anoxic nitrite reduction. The reaction suggests a new pathway for fast NO delivery under hypoxia, precisely when the vasodilating properties of nitric oxide are most needed. (c) 2006 Elsevier Inc. All rights reserved. (10.1016/j.bbrc.2006.01.031)
  DOI : 10.1016/j.bbrc.2006.01.031
• Role of the middle residue in the triple tryptophan electron transfer chain of DNA photolyase: ultrafast spectroscopy of a Trp→Phe mutant
  
  • Lukacs Andras
  • Eker A.P.M.
  • Byrdin M.
  • Villette Sandrine
  • Pan J.
  • Brettel K.
  • Vos Marten H.
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110 (32), pp.15654-15658.
• Mechanisms involved in the swelling of erythrocytes caused by Pacific and Caribbean ciguatoxins
  
  • Sauviat Martin-Pierre
  • Boydron-Le Garrec Raphaële
  • Masson Jean-Baptiste
  • Lewis Richard L.
  • Vernoux Jean-Paul
  • Molgó Jordi
  • Laurent Dominique
  • Benoit Evelyne
  Blood Cells, Molecules and Diseases, Elsevier, 2006, 36(1), pp.1-9. The mechanisms underlying the swelling of frog red blood cells (RBC), induced by Pacific (P-CTX-1) and Caribbean (C-CTX-1) ciguatoxins (CTXs), were investigated by measuring the length, width and surface of their elliptic shape. P-CTX-1 (0.5 to 5 nM) and C-CTX-1 (1 nM) induced RBC swelling within 60 min. The CTXs-induced RBC swelling was blocked by apamin (1 μM) and by Sr2+ (1 mM). P-CTX-1-induced RBC swelling was prevented and inhibited by H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (27 μM), an inhibitor of soluble guanylate cyclase (sGC), and NOS blockade by NG methyl-l-arginine (l-NMA; 10 μM). Cytochalasin D (cytD, 10 μM) increased RBC surface and mimicked CTX effect but did not prevent the P-CTX-1-induced l-NMA-sensitive extra increase. Calculations revealed that P-CTX-1 and cytD increase RBC total surface envelop and volume. These data strongly suggest that the molecular mechanisms underlying CTXs-induced RBC swelling involve the NO pathway by an activation of the inducible NOS, leading to sGC activation which modulates intracellular cGMP and regulates L-type Ca2+ channels. The resulting increase in intracellular Ca2+ content, in turn, disrupts the actin cytoskeleton, which causes a water influx and triggers a Ca2+-activated K+ current through SK2 isoform channels. (10.1016/j.bcmd.2005.10.007)
  DOI : 10.1016/j.bcmd.2005.10.007
• Photoinduced electron transfer from a novel nanotrigger addressed to the NADPH site within the endothelial NO-synthase to the flavin moieties of the protein
  
  • Beaumont Edward
  • Robin Anne-Claire
  • Berka Vladimir
  • Tsai Ah-Lim
  • Blanchard-Desce Mireille
  • Lambry Jean-Christophe
  • Slama-Schwok Anny
  Nitric Oxide: Biology and Chemistry, Elsevier, 2006, 14 (4), pp.14-15. We developed a new selective molecular tool to trigger enzymatic activity in a synchronous manner and monitor the sequence of the kinetic events by ultra-fast transient spectroscopy. Our approach is based on a synthetic nanotrigger addressing a selected site within proteins, namely the conserved NADPH binding site common to many enzymes involved in bioreductive processes [1]. The nanotrigger combines a "docking" subunit responsible for the recognition of NADPH sites within proteins and a "chromophoric" subunit responsive to light excitation and able to transfer electrons to the flavin moieties of proteins. We present the first spectroscopic data on such a nanotrigger [1] in the presence of the reductase domain of the endothelial nitric oxide synthase eNOSred. (10.1016/j.niox.2006.04.050)
  DOI : 10.1016/j.niox.2006.04.050
• Nouvelle approche des fibroses par microscopie multiphotonique avec génération de second harmonique [New approach of fibrosis by multiphoton microscopy with second harmonic generation]
  
  • Hernest Monica
  • Pena Ana-Maria
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Tharaux Pierre-Louis
  Médecine/Sciences, EDP Sciences, 2006, 22 (10), pp.820-821. La fibrose est une réponse adaptative pathologique qui détruit non spécifiquement les tissus. Il s'agit d'un processus universel de réparation des tissus qui survient en réaction à de nombreux types d'agressions telles les contraintes mécaniques, les brûlures, les radiations ionisantes, l'ischémie, l'inflammation. Ces agressions concourent de manière intriquée à la physiopathologie des maladies infectieuses, tumorales ou auto-immunes, de l'hypertension artérielle et des maladies cardio-vasculaires. Le terme de fibrose décrit précisément l'accumulation nouvelle de protéines de la matrice extra-cellulaire selon un arrangement spatial fibrillaire caractéristique. Il s'agit essentiellement de molécules de collagènes de type I et III (voire de type II, V ou XI) synthétisées sous forme de triples hélices elles-mêmes assemblées en fibrilles par les cellules fibroblastiques. L'apparition des collagènes fibrillaires marque un changement qualitatif et quantitatif de composition des collagènes des tissus. Réciproquement, ces changements de la matrice extracellulaire influencent le phénotype des cellules qui y résident. Ainsi dans le rein normal, le collagène de type I n'existe que dans l'adventice artériel. Son apparition au sein des autres structures de cet organe marque une fibrose tubulo-interstitielle qui constitue le meilleur marqueur pronostic défavorable d'une évolution vers l'insuffisance rénale terminale, et ce quelle que soit la maladie causale. Ainsi, comme lors des fibroses compliquant les hépatopathies et les pneumopathies chroniques, les séquelles de brûlures ou d'abrasions cutanées-muqueuses ou encore le remodelage cardiaque et vasculaire, le réarrangement de la géométrie de la matrice extracellulaire altère l'organisation fonctionnelle du tissu considéré. De ce fait, ce processus de réparation a des effets fonctionnels délétères qui constituent un enjeu médical majeur. Les fibrilles de collagène ont des capacités d'auto-assemblage qui sont aussi catalysées et stabilisées ou au contraire empêchées par les enzymes de la matrice extracellulaire. Le développement de la fibrose ou sa régression dépend donc ainsi du bilan des équilibres biologiques de ces mécanismes. Il est donc crucial de caractériser les changements extracellulaires et cellulaires qui font du restutio ad integrum de l'architecture et de la fonction tissulaire un défi biomédical. (10.1051/medsci/20062210820)
  DOI : 10.1051/medsci/20062210820
• Nonlinear microscopy using Second Harmonic Generation from myosin filaments and fibrillar collagens
  
  • Pena Ana-Maria
  • Strupler Mathias
  • Boulesteix Thierry
  • Fabre Aude
  • Hernest Monica
  • Tharaux Pierre-Louis
  • Crestani Bruno
  • Martin Jean-Louis
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  , 2006.
• La transglutaminase tissulaire promeut l'organisation fibrillaire du collagène et la progression de la fibrose rénale hypertensive dépendante de l'angiotensine II. (sélectionné pour le prix poster)
  
  • Hernest Monica
  • Pena Ana-Maria
  • Beaurepaire Emmanuel
  • Chatziantoniou C.
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  , 2006.
• Optical in situ size determination of single lanthanide-ion doped oxide nanoparticles
  
  • Casanova Didier
  • Giaume Domitile
  • Beaurepaire Emmanuel
  • Gacoin Thierry
  • Boilot Jean-Pierre
  • Alexandrou Antigoni
  Applied Physics Letters, American Institute of Physics, 2006, 89 (25), pp.253103. We show that the size of a lanthanide-ion doped nanoparticle can be accurately determined from its luminosity. The optically determined size distribution is in very good agreement with the distribution obtained from transmission electron microscopy. These data confirm that single nanoparticles are visualized in microscopy experiments. Nanoparticles as small as 13 nm are detectable with integration times of 500 ms. (c) 2006 American Institute of Physics. (10.1063/1.2405871)
  DOI : 10.1063/1.2405871
• True near field versus contrast near field imaging.
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14 (24), pp.11566-11574. We demonstrate that in near field imaging, interaction between light and sample can be divided into two main areas: the true near field and the contrast near field domain. We performed extensive numerical simulations in order to identify the limits of these areas, and to investigate contrast near field imaging in which much easier propagation calculation can be achieved. Finally, we show an application with terahertz axonal imaging. © 2006 Optical Society of America (10.1364/OE.14.011566)
  DOI : 10.1364/OE.14.011566
• A Hydrophobic Distal Pocket Affects NO-heme Geminate Recombination Dynamics in Dehaloperoxidase and H64V Myoglobin
  
  • Franzen Stefan
  • Jasaitis Audrius
  • Belyea Jennifer
  • Brewer Scott
  • Casey Romain
  • Macfarlane a W Iv
  • Stanley R.
  • Vos Marten H.
  • Martin Jean-Louis
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110, pp.14483-14493.
• Interferometric Fourier transform coherent anti-stokes Raman scattering
  
  • Cui M.
  • Joffre Manuel
  • Skodack J.
  • Ogilvie Jennifer P.
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14 (18), pp.8448-8458. We present an interferometric time-domain Fourier transform implementation of coherent anti-Stokes Raman scattering (CARS). Based on a single femtosecond laser source, the method provides a straight-forward scheme for obtaining high resolution CARS spectra. We give a theoretical description of the method, and demonstrate good agreement between simulation and experimental CARS spectra. We also discuss the method's relation to other CARS approaches for microscopy and microspectroscopy applications. Cop. 2006 Optical Society of America. (10.1364/OE.14.008448)
  DOI : 10.1364/OE.14.008448
• Dynamics of NO rebinding to the heme domain of NO synthase-like proteins from bacterial pathogens
  
  • Gautier Christian
  • Martasek P.
  • Mikula I.
  • Nioche P.
  • Raman C.S.
  • Slama-Schwok Anny
  Nitric Oxide, 2006, 15 (4), pp.312. Some Gram-positive bacterial pathogens harbor a gene that encodes a protein (HNS, Heme domain of NO Synthase-like proteins) with striking sequence identity to the oxygenase domain of mammalian NO synthases (NOS). However, they lack the N-terminal and the Zn-cysteine motif participating to the stability of an active dimer in the mammalian isoforms. The unique properties of HNS make it an excellent model system for probing how the heme environment tunes NO dynamics and for comparing it to the endothelial NO synthase heme domain (eNOSHD) using ultrafast transient spectroscopy. NO rebinding in HNS from Staphylococcus aureus (SA-HNS) is faster than that measured for either Bacillus anthracis (BA-HNS) or for eNOSHD in both oxidized and reduced forms in the presence of arginine. To test whether these distinct rates arise from different energy barriers for NO recombination, we measured rebinding kinetics at several temperatures. Our data are consistent with different barriers for NO recombination in SA-HNS and BA-HNS and the presence of a second NO-binding site. The hypothesis that an additional NO-binding cavity is present in BA-HNS is also consistent with the effect of the NO concentration on its rebinding. The lack of the effect of NO concentration on the geminate rebinding in SA-HNS could be due to an isolated second site. We confirm the existence of a second NO site in the oxygenase domain of the reduced eNOS as previously hypothesized [A. Slama-Schwok, M. Négrerie, V. Berka, J.C. Lambry, A.L. Tsai, M.H. Vos, J.L. Martin, Nitric oxide (NO) traffic in endothelial NO synthase. Evidence for a new NO binding site dependent on tetrahydrobiopterin? J. Biol. Chem. 277 (2002) 7581-7586]. This site requires the presence of arginine and BH4; and we propose that NO dynamic and escape from eNOS is regulated by the active site H-bonding network connecting between the heme, the substrate, and cofactor. Cop. 2006 Elsevier Inc. All rights reserved. (10.1016/j.niox.2006.03.010)
  DOI : 10.1016/j.niox.2006.03.010
• Ultrafast Conformational Changes in Carboxy-Myoglobin Studied by Time-Resolved Circular Dichroism
  
  • Dartigalongue Thibault
  • Hache François
  , 2006.
• Time-resolved circular dichroism in carbonmonoxy-myoglobin: The central role of the proximal histidine
  
  • Dartigalongue Thibault
  • Hache François
  Chirality, Wiley, 2006, 18 (4), pp.273. A calculation of the circular dichroism (CD) spectra of carbonmonoxy-and deoxy-myoglobin is carried out in relation to a time-resolved CD experiment. This calculation allows us to assign a dominant role to the proximal histidine in the definition of the electronic normal modes and to interpret the transient CD structure observed in a strain of the proximal histidine. This strain builds up in 10 ps and relaxes in 50 ps as the protein evolves towards its deoxy form. Cop. 2006 Wiley-Liss, Inc. (10.1002/chir.20254)
  DOI : 10.1002/chir.20254
• Role of heme iron coordination and protein structure in the dynamics and geminate rebinding of nitric oxide to the H93G myoglobin mutant: Implications for nitric oxide sensors
  
  • Négrerie Michel
  • Kruglik Sergei G.
  • Lambry Jean-Christophe
  • Vos Marten H.
  • Martin Jean-Louis
  • Franzen S.
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281, pp.10389. The influence of the heme iron coordination on nitric oxide binding dynamics was investigated for the myoglobin mutant H93G (H93G-Mb) by picosecond absorption and resonance Raman time-resolved spectroscopies. In the H93G-Mb, the glycine replacing the proximal histidine does not interact with the heme iron so that exogenous substituents like imidazole may coordinate to the iron at the proximal position. Nitrosylation of H93G-Mb leads to either 6- or 5-coordinate species depending on the imidazole concentration. At high concentrations, (imidazole)-(NO)-6-coordinate heme is formed, and the photoinduced rebinding kinetics reveal two exponential picosecond phases (~10 and ~100 ps) similar to those of wild type myoglobin. At low concentrations, imidazole is displaced by the trans effect leading to a (NO)-5-coordinate heme, becoming 4-coordinate immediately after photolysis as revealed from the transient Raman spectrum. In this case, NO rebinding kinetics remain bi-exponential with no change in time constant of the fast component whose amplitude increases with respect to the 6-coordinate species. Bi-exponential NO geminate rebinding in 5-coordinate H93G-Mb is in contrast with the single-exponential process reported for nitrosylated soluble guanylate cyclase (Negrerie, M., Bouzhir, L., Martin, J. L., and Liebl, U. (2001) J. Biol. Chem. 276, 46815-46821). Thus, our data show that the iron coordination state or the heme iron out-of-plane motion are not at the origin of the bi-exponential kinetics, which depends upon the protein structure, and that the 4-coordinate state favors the fast phase of NO geminate rebinding. Consequently, the heme coordination state together with the energy barriers provided by the protein structure control the dynamics and affinity for NO-binding enzymes. Cop. 2006 by The American Society for Biochemistry and Molecular Biology, Inc. (10.1074/jbc.M513375200)
  DOI : 10.1074/jbc.M513375200
• Sensitivity of cardiac background inward rectifying K+ outward current (IK1) to the alkaloids lepadiformines A, B, and C
  
  • Sauviat Martin-Pierre
  • Vercauteren J.
  • Grimaud N.
  • Jugé M.
  • Nabil M.
  • Petit J.-Y.
  • Biard J.-F.
  Journal of Natural Products, American Chemical Society, 2006, 69 (4), pp.558. Three marine alkaloids, purified from Clavelina moluccensis, were structurally identified as lepadiformines A, B, and C and studied on frog atrial myocytes IK1, using the patch-clamp technique. Lepadiformine A (0.4 to 3.3 μM) blocked IK1 dose-dependently with an apparent dissociation constant (KD) equal to 1.42 μM and a stoichiometry of 0.77. The block is voltage-dependent, suggesting that lepadiformine A occupies a receptor site located at about two-thirds of the membrane depth. The shortening of the aliphatic chain at position C13 of lepadiformine B decreased the potency of the molecule to block IK1 but not the affinity (KD = 1.56 μM) and stoichiometry (0.72). Additional deletion of the oxygenated side chain at C2 in lepadiformine C markedly decreased the inhibitory effect of the molecule. In conclusion, lepadiformine modulates IK1 response in cardiac muscle. The oxygenated side chain in C2 is implicated in the affinity of lepadiformine, which behaved as an amine, for a receptor located near or inside the IK1 pore, and the aliphatic chain length at position C13 is involved in the degree of IK1 blockage. (10.1021/np050215s)
  DOI : 10.1021/np050215s
• Generation and complete characterization of intense mid-infrared ultrashort pulses
  
  • Ventalon Cathie
  • Fraser James M.
  • Likforman Jean-Pierre
  • Villeneuve D. M.
  • Corkum Paul B.
  • Joffre Manuel
  Journal of the Optical Society of America B, Optical Society of America, 2006, 23, pp.332.
• SHG microscopy. Quantitative scoring of kidney collagen fibrosis. (prix du meilleur poster)
  
  • Strupler Mathias
  • Hernest Monica
  • Pena Ana-Maria
  • Martin Jean-Louis
  • Beaurepaire Emmanuel
  • Tharaux Pierre-Louis
  • Schanne-Klein Marie-Claire
  , 2006.
• Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy.
  
  • Débarre Delphine
  • Supatto Willy
  • Pena Ana-Maria
  • Fabre Aurelie J
  • Tordjmann Thierry
  • Combettes Laurent
  • Schanne-Klein Marie-Claire
  • Beaurepaire Emmanuel
  Nature Methods, Nature Publishing Group, 2006, 3 (1), pp.47-53. Lipid bodies have an important role in energy storage and lipid regulation. Here we show that lipid bodies are a major source of contrast in third-harmonic generation (THG) microscopy of cells and tissues. In hepatocytes, micrometer-sized lipid bodies produce a THG signal 1-2 orders of magnitude larger than other structures, which allows one to image them with high specificity. THG microscopy with approximately 1,200 nm excitation can be used to follow the distribution of lipid bodies in a variety of unstained samples including insect embryos, plant seeds and intact mammalian tissue (liver, lung). We found that epi-THG imaging is possible in weakly absorbing tissues because bulk scattering redirects a substantial fraction of the forward-generated harmonic light toward the objective. Finally, we show that the combination of THG microscopy with two-photon and second-harmonic imaging provides a new tool for exploring the interactions between lipid bodies, extracellular matrix and fluorescent compounds (vitamin A, NADH and others) in tissues. (10.1038/nmeth813)
  DOI : 10.1038/nmeth813